beta Actin antibody

Catalog No. ABIN654127

This Rabbit Polyclonal antibody specifically detects beta Actin in WB, IHC (p) and FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for beta Actin antibody (ABIN654127)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB23152

Product Details anti-beta Actin Antibody

Specificity: This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB.

Predicted Reactivity: Cow (Bovine),Chicken,Mouse (Murine),Pig (Porcine),Monkey,Rat (Rattus),Xenopus laevis

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ACTB antibody is generated from rabbits immunized with a recombinant protein from human ACTB.

Isotype: Ig Fraction

Application Details

Application Notes: WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-beta Actin antibody (ABIN654127)

Liu, Peng, Zhong, Liu, Song, Wang: "Effect of 5-caffeoylquinic acid on the NF-?B signaling pathway, peroxisome proliferator-activated receptor gamma 2, and macrophage infiltration in high-fat diet-fed Sprague-Dawley rat adipose tissue." in: Food & function, Vol. 6, Issue 8, pp. 2779-86, (2015) (PubMed).

Shrestha, Mousa, Heintz: "Layer 2/3 pyramidal cells in the medial prefrontal cortex moderate stress induced depressive behaviors." in: eLife, Vol. 4, (2015) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB

Background: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Synonyms: Actin, cytoplasmic 1,ACTB,

Molecular Weight: 41737 DA

Gene ID: 60

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction